Red-finned blue-eye (Scaturiginichthys vermeilipinnis) inhabit just nine Great Artesian Basin springs on Bush Heritage Australia’s Edgbaston Nature Refuge, Western Queensland. The persistence of this species is threatened by Gambusia holbrooki which can invade springs during flooding events, leading to local extirpation within 3 – 5 years. The implementation of a multi-pronged conservation approach is underway to save this critically endangered species. Strategies include Gambusia exclusion fencing which prevents the movement of fish into springs during flooding, translocation of captive bred red-finned blue-eye into springs which are free from Gambusia and an off-site insurance population. Through these efforts population decline has halted. Moving forward with a focus on genetic management of translocated populations we will work to ensure the viability and persistence of these populations.