Waterholes are crucial to native fauna and indigenous peoples in central Australia, providing refuge and resources in an otherwise dry landscape. This research investigated the use of two culturally important waterholes by terrestrial vertebrate fauna near Ltyentye Apurte (Santa Teresa): Salt and Hayes Springs. Camera traps were used to capture images of fauna visiting the waterholes between 2016 and 2017. Seasonal variation in visitation was evaluated using the local indigenous calendar (that includes 7 seasons). Waterholes were heavily used by native fauna, but visitation varied between seasons. Our study supports the importance of waterhole conservation.